Real-Time 3D Microscopy for Label-Free Analysis
The Problem We Solve
Traditionally, you had to choose between high-quality microscopy and high-throughput analysis of colloids.
Holloid delivers both.
Our Technology
In-line 3D holography
Light passes through a sample, and an image is recorded that contains holograms—i.e., 3D image information.
Numerical back-propagation
Algorithms generate a stack of bright-field images that include phase information.
Contrast simulation & novel algorithms
This information is used to simulate different contrast methods, including new and advanced ones.
Object characterization
All objects in a sample volume can be detected and characterized across multiple parameters.
Dynamic tracking of all objects
Objects tracked over time can be dynamically characterized using various metrics.
Why It's Better
Our technology creates sharper images with higher contrast and can therefore discriminate particles that others miss. It’s real-time, label-free 3D microscopy, which enables high-throughput analysis and continuously captures millions of objects. Thanks to our precise algorithms, you can get actionable data faster.
What We Quantify
Holloid provides real-time multi-dimensional data on physical characteristics. We can quantify the following physical properties in real-time for all objects based on 3D imaging with the throughput of cytometry.
Static
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Number
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Size
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Shape
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Amount of scattered light
(dry mass) -
Inner structure (e.g., centrosome, inclusion bodies)
Dynamic
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Motility
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Translational diffusion (hydrodynamic size)
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Rotational diffusion
(asymmetric objects) -
Sedimentation rate (density)
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Interaction potentials
Hollometer
The devices are the size of a shoebox and can be placed on-premises for continuous process monitoring.
Size, shape, internal structure, motility, and other characteristics and dynamics of each object are measured continuously.
The data can be automatically processed on the premises or in the cloud.
