Real-Time 3D Microscopy for Label-Free Analysis
The Problem We Solve
Traditionally, you had to choose between high-quality microscopy and high-throughput analysis of colloids.
Holloid delivers both.
Our Technology
In-line 3D holography
Light passes through a sample, and an image is recorded that contains holograms—i.e., 3D image information.
Numerical back-propagation
Algorithms generate a stack of bright-field images that include phase information.
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Contrast simulation & novel algorithms
This information is used to simulate different contrast methods, including new and advanced ones.
Object characterization
All objects in a sample volume can be detected and characterized across multiple parameters.
Dynamic tracking of all objects
Objects tracked over time can be dynamically characterized using various metrics.
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Why It's Better
Our technology creates sharper images with higher contrast and can therefore discriminate particles that others miss. It’s real-time, label-free 3D microscopy, which enables high-throughput analysis and continuously captures millions of objects. Thanks to our precise algorithms, you can get actionable data faster.
What We Quantify
Holloid provides real-time multi-dimensional data on physical characteristics. We can quantify the following physical properties in real-time for all objects based on 3D imaging with the throughput of cytometry.
Static
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Number
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Size
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Shape
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Amount of scattered light
(dry mass) -
Inner structure (e.g., centrosome, inclusion bodies)
Dynamic
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Motility
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Translational diffusion (hydrodynamic size)
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Rotational diffusion
(asymmetric objects) -
Sedimentation rate (density)
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Interaction potentials
Hollometer
The devices are the size of a shoebox and can be placed on-premises for continuous process monitoring.
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​Size, shape, internal structure, motility, and other characteristics and dynamics of each object are measured continuously.
The data can be automatically processed on the premises or in the cloud.
