Microscope

Our technology made simple

3D imaging of each and all colloidal objects

The problem we solve:

When analysing colloids, one used to have to choose between information quality and high throughput. Not with Holloid !

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Our technology
In-line phase-contrast holography  

  1. Recording defocussed images

  2. Numerical ‘back-propagation’ generates a stack of pictures

    • representing the light-field in 3D

    • including phase information

  3. This information is used to simulate different contrast methods, including novel ones

  4. From these, all the objects in a sample volume can be found and characterized

  5. The objects can be tracked in space and the dynamics can be characterized by applying this to a movie or live footage in real-time

Sharper and faster images

  1. Sharper and higher contrast images of the objects than with current technologies

  2. We can filter and discriminate particulates in colloidal media that others cannot

  3. Our fast algorithms allow to analyze the information in real-time

Our in-line phase-contrast holography enables

real-time       cytometric analysis

using

label-free       3D microscopy

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See more than ever before: 
Static and dynamic properties

Holloid provides real-time multi-dimensional data on physical characteristics. We can quantify the following physical properties in real-time for all objects:

  • Static properties (require snapshots only):

    • number

    • size

    • shape

    • eccentricity

    • amount of scattered light (dry mass)

    • inner structure (of cells: centrosome, inclusion bodies, etc.)

  • Dynamic properties (require multi-image acquisition, “movies”):

    • translational diffusion (hydrodynamic size)

    • rotational diffusion (asymmetric objects)

    • sedimentation rate (density)

    • interaction potentials

including motion and interactions, of cells and other colloids, based on 3D imaging, with the throughput of cytometry.

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