Big data
on small things
Our technology made simple
3D imaging of each and all colloidal objects
The problem we solve:
When analysing colloids, one used to have to choose between information quality and high throughput. Not with Holloid !
Our technology
In-line phase-contrast holography
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Recording defocussed images
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Numerical ‘back-propagation’ generates a stack of pictures
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representing the light-field in 3D
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including phase information
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This information is used to simulate different contrast methods, including novel ones
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From these, all the objects in a sample volume can be found and characterized
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The objects can be tracked in space and the dynamics can be characterized by applying this to a movie or live footage in real-time
Sharper and faster images
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Sharper and higher contrast images of the objects than with current technologies
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We can filter and discriminate particulates in colloidal media that others cannot
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Our fast algorithms allow to analyze the information in real-time
Our in-line phase-contrast holography enables
real-time cytometric analysis
using
label-free 3D microscopy
See more than ever before:
Static and dynamic properties
Holloid provides real-time multi-dimensional data on physical characteristics. We can quantify the following physical properties in real-time for all objects:
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Static properties (require snapshots only):
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number
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size
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shape
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eccentricity
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amount of scattered light (dry mass)
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inner structure (of cells: centrosome, inclusion bodies, etc.)
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Dynamic properties (require multi-image acquisition, “movies”):
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translational diffusion (hydrodynamic size)
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rotational diffusion (asymmetric objects)
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sedimentation rate (density)
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interaction potentials
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including motion and interactions, of cells and other colloids, based on 3D imaging, with the throughput of cytometry.